bio rad uno q 12 column (Bio-Rad)
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bio rad uno q 12 column
Bio Rad Uno Q 12 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+uno+q+12+column/UNO+Q12+Column/bio_rxiv__2025__07__17__665390-154-35-35
Average 94 stars, based on 96 article reviews
Bio Rad Uno Q 12 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+uno+q+12+column/UNO+Q12+Column/bio_rxiv__2025__07__17__665390-154-35-35
Average 94 stars, based on 96 article reviews
bio rad uno q 12 column - by Bioz Stars,
2026-10
94/100 stars
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Purification:Article Title: Cryo-EM structures of naturally occurring dimeric photosystem II complexes lacking the Mn 4 CaO 5 cluster Article Snippet: .. The solubilized PSII was purified by nickel affinity chromatography followed by anion exchange chromatography according to Nowaczyk et al with the following modifications: PSII complexes eluted from nickel affinity column were loaded directly on the Article Title: Crystal structure of CyanoQ from the thermophilic cyanobacterium Thermosynechococcus elongatus and detection in isolated photosystem II complexes Article Snippet: These membranes were then used to isolate PSII oxygen-evolving complexes from WT T. elongatus using the two-step anion-exchange chromatography procedure described by Kern et al. ( ). .. Dimeric His-tagged oxygen-evolving complexes were isolated from a His-tagged CP47 strain of T. elongatus by Ni-affinity purification followed by anion-exchange chromatography as described by Nowaczyk et al. ( ) except for the following modifications: freshly grown cells were broken in 20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 10 % (v/v) glycerol and 1.2 M betaine, and unbroken cells were removed by centrifuging at 1,000 g (JA14 rotor, Beckman Coulter Ltd.) for 5 min at 4 °C; the resulting supernatant was diluted to a Chl concentration of 1 mg/ml and the thylakoid membranes were solubilised for 10 min at 4 °C with 1 % (w/v) n-dodecyl-β- D -maltoside (β-DDM) at a detergent to Chl ratio of 18:1 followed by a 30-min spin at 4 °C and 184,000 g (Ti70 rotor, Beckman Coulter Ltd.); the extract was incubated for 45 min with Ni-affinity resin (Probond Resin, Invitrogen) equilibrated in buffer E (20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 0.5 M D-mannitol and 0.03 % (w/v) β-DDM); after loading, the Ni-affinity column was washed with 6 column volumes of buffer E + 5-mM histidine; His-tagged PSII complexes were eluted by application of a 100-mM histidine isocratic step gradient in buffer E and loaded directly onto a Affinity Chromatography:Article Title: Cryo-EM structures of naturally occurring dimeric photosystem II complexes lacking the Mn 4 CaO 5 cluster Article Snippet: .. The solubilized PSII was purified by nickel affinity chromatography followed by anion exchange chromatography according to Nowaczyk et al with the following modifications: PSII complexes eluted from nickel affinity column were loaded directly on the Chromatography:Article Title: Cryo-EM structures of naturally occurring dimeric photosystem II complexes lacking the Mn 4 CaO 5 cluster Article Snippet: .. The solubilized PSII was purified by nickel affinity chromatography followed by anion exchange chromatography according to Nowaczyk et al with the following modifications: PSII complexes eluted from nickel affinity column were loaded directly on the Article Title: Crystal structure of CyanoQ from the thermophilic cyanobacterium Thermosynechococcus elongatus and detection in isolated photosystem II complexes Article Snippet: These membranes were then used to isolate PSII oxygen-evolving complexes from WT T. elongatus using the two-step anion-exchange chromatography procedure described by Kern et al. ( ). .. Dimeric His-tagged oxygen-evolving complexes were isolated from a His-tagged CP47 strain of T. elongatus by Ni-affinity purification followed by anion-exchange chromatography as described by Nowaczyk et al. ( ) except for the following modifications: freshly grown cells were broken in 20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 10 % (v/v) glycerol and 1.2 M betaine, and unbroken cells were removed by centrifuging at 1,000 g (JA14 rotor, Beckman Coulter Ltd.) for 5 min at 4 °C; the resulting supernatant was diluted to a Chl concentration of 1 mg/ml and the thylakoid membranes were solubilised for 10 min at 4 °C with 1 % (w/v) n-dodecyl-β- D -maltoside (β-DDM) at a detergent to Chl ratio of 18:1 followed by a 30-min spin at 4 °C and 184,000 g (Ti70 rotor, Beckman Coulter Ltd.); the extract was incubated for 45 min with Ni-affinity resin (Probond Resin, Invitrogen) equilibrated in buffer E (20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 0.5 M D-mannitol and 0.03 % (w/v) β-DDM); after loading, the Ni-affinity column was washed with 6 column volumes of buffer E + 5-mM histidine; His-tagged PSII complexes were eluted by application of a 100-mM histidine isocratic step gradient in buffer E and loaded directly onto a Affinity Column:Article Title: Cryo-EM structures of naturally occurring dimeric photosystem II complexes lacking the Mn 4 CaO 5 cluster Article Snippet: .. The solubilized PSII was purified by nickel affinity chromatography followed by anion exchange chromatography according to Nowaczyk et al with the following modifications: PSII complexes eluted from nickel affinity column were loaded directly on the Activity Assay:Article Title: Identification of a novel subunit of respiratory complex I from Thermus thermophilus. Article Snippet: The hydrophilic domain (peripheral arm) of the proton-translocating NADH:quinone oxidoreductase (complex I) from the thermophilic organism Thermus thermophilus HB8 has been purified and characterized.. The subcomplex is stable in sodium dodecyl sulfate up to 80 °C.. Of nine iron-sulfur clusters, four to five (one or two binuclear and three tetranuclear) could be detected by EPR in the NADHreduced enzyme. Isolation:Article Title: Crystal structure of CyanoQ from the thermophilic cyanobacterium Thermosynechococcus elongatus and detection in isolated photosystem II complexes Article Snippet: These membranes were then used to isolate PSII oxygen-evolving complexes from WT T. elongatus using the two-step anion-exchange chromatography procedure described by Kern et al. ( ). .. Dimeric His-tagged oxygen-evolving complexes were isolated from a His-tagged CP47 strain of T. elongatus by Ni-affinity purification followed by anion-exchange chromatography as described by Nowaczyk et al. ( ) except for the following modifications: freshly grown cells were broken in 20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 10 % (v/v) glycerol and 1.2 M betaine, and unbroken cells were removed by centrifuging at 1,000 g (JA14 rotor, Beckman Coulter Ltd.) for 5 min at 4 °C; the resulting supernatant was diluted to a Chl concentration of 1 mg/ml and the thylakoid membranes were solubilised for 10 min at 4 °C with 1 % (w/v) n-dodecyl-β- D -maltoside (β-DDM) at a detergent to Chl ratio of 18:1 followed by a 30-min spin at 4 °C and 184,000 g (Ti70 rotor, Beckman Coulter Ltd.); the extract was incubated for 45 min with Ni-affinity resin (Probond Resin, Invitrogen) equilibrated in buffer E (20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 0.5 M D-mannitol and 0.03 % (w/v) β-DDM); after loading, the Ni-affinity column was washed with 6 column volumes of buffer E + 5-mM histidine; His-tagged PSII complexes were eluted by application of a 100-mM histidine isocratic step gradient in buffer E and loaded directly onto a Concentration Assay:Article Title: Crystal structure of CyanoQ from the thermophilic cyanobacterium Thermosynechococcus elongatus and detection in isolated photosystem II complexes Article Snippet: These membranes were then used to isolate PSII oxygen-evolving complexes from WT T. elongatus using the two-step anion-exchange chromatography procedure described by Kern et al. ( ). .. Dimeric His-tagged oxygen-evolving complexes were isolated from a His-tagged CP47 strain of T. elongatus by Ni-affinity purification followed by anion-exchange chromatography as described by Nowaczyk et al. ( ) except for the following modifications: freshly grown cells were broken in 20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 10 % (v/v) glycerol and 1.2 M betaine, and unbroken cells were removed by centrifuging at 1,000 g (JA14 rotor, Beckman Coulter Ltd.) for 5 min at 4 °C; the resulting supernatant was diluted to a Chl concentration of 1 mg/ml and the thylakoid membranes were solubilised for 10 min at 4 °C with 1 % (w/v) n-dodecyl-β- D -maltoside (β-DDM) at a detergent to Chl ratio of 18:1 followed by a 30-min spin at 4 °C and 184,000 g (Ti70 rotor, Beckman Coulter Ltd.); the extract was incubated for 45 min with Ni-affinity resin (Probond Resin, Invitrogen) equilibrated in buffer E (20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 0.5 M D-mannitol and 0.03 % (w/v) β-DDM); after loading, the Ni-affinity column was washed with 6 column volumes of buffer E + 5-mM histidine; His-tagged PSII complexes were eluted by application of a 100-mM histidine isocratic step gradient in buffer E and loaded directly onto a Incubation:Article Title: Crystal structure of CyanoQ from the thermophilic cyanobacterium Thermosynechococcus elongatus and detection in isolated photosystem II complexes Article Snippet: These membranes were then used to isolate PSII oxygen-evolving complexes from WT T. elongatus using the two-step anion-exchange chromatography procedure described by Kern et al. ( ). .. Dimeric His-tagged oxygen-evolving complexes were isolated from a His-tagged CP47 strain of T. elongatus by Ni-affinity purification followed by anion-exchange chromatography as described by Nowaczyk et al. ( ) except for the following modifications: freshly grown cells were broken in 20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 10 % (v/v) glycerol and 1.2 M betaine, and unbroken cells were removed by centrifuging at 1,000 g (JA14 rotor, Beckman Coulter Ltd.) for 5 min at 4 °C; the resulting supernatant was diluted to a Chl concentration of 1 mg/ml and the thylakoid membranes were solubilised for 10 min at 4 °C with 1 % (w/v) n-dodecyl-β- D -maltoside (β-DDM) at a detergent to Chl ratio of 18:1 followed by a 30-min spin at 4 °C and 184,000 g (Ti70 rotor, Beckman Coulter Ltd.); the extract was incubated for 45 min with Ni-affinity resin (Probond Resin, Invitrogen) equilibrated in buffer E (20 mM MES–NaOH pH 6.5, 2.5 mM CaCl 2 , 2.5 mM MgCl 2 , 0.5 M D-mannitol and 0.03 % (w/v) β-DDM); after loading, the Ni-affinity column was washed with 6 column volumes of buffer E + 5-mM histidine; His-tagged PSII complexes were eluted by application of a 100-mM histidine isocratic step gradient in buffer E and loaded directly onto a |